SLA-DOB及CD4基因新突变显著影响长白妊娠母猪的血常规性状

doi:10.11843/j.issn.0366-6964.2017.11.005

摘要/Abstract

摘要：

旨在寻找影响妊娠母猪血常规性状的单核苷酸多态性（SNP）位点及分子标记。选用SLA-DOB、CD4作为候选基因，采集358头长白妊娠母猪外周血血样，检测血常规19项指标，并对候选基因的多态位点与血常规指标进行关联分析。结果发现，在检测到的12个遗传变异位点中，SLA-DOB基因的SNP1、SNP2、SNP3和CD4基因的Ins9（Insertion 9）、SNP10、SNP11为新发现的变异位点。其中，SNP1与血小板平均体积（MPV）关联极显著（P<0.01），与红细胞压积（HCT）和红细胞分布宽度SD （RDW_SD）均关联显著（P<0.05）；SNP2与MPV关联极显著（P<0.01）；SNP3与平均血红蛋白含量（MCH）和平均血红蛋白浓度（MCHC）均关联显著（P<0.05）；Ins9与红细胞平均体积（MCV）关联显著（P<0.05），与MPV关联极显著（P<0.01）；SNP10和SNP11均与MCV、MCH及MPV关联极显著（P<0.01），并均与RDW_SD关联显著（P<0.05），另外，SNP10与MCHC关联显著（P<0.05），表明这些新突变为影响妊娠母猪免疫力潜在的重要多态位点。结果提示，SLA-DOB及CD4基因及其突变位点可作为妊娠母猪免疫力性状的重要候选基因及关键分子标记。

Abstract:

This study aimed to find single nucleotide polymorphism (SNP) sites and molecular markers affecting the routine blood traits of pregnant sows. Porcine SLA-DOB and CD4 were chosen as candidate genes, blood samples were randomly collected from 358 pregnant Landrace sows and used to detect 19 routine blood indexes, then association analysis was conducted between the polymorphic sites of candidate genes and the routine blood traits.We found that 12 mutations, in which SNP1, SNP2 and SNP3 of SLA-DOB gene as well as Ins9, SNP10 and SNP11 in CD4 gene were all newly discovered mutations. SNP1 was significantly associated with mean platelet volume (MPV) (P<0.01), hematocrit (HCT) and red cell volume distribution width SD (RDW_SD) (P<0.05); SNP2 was extremely significantly associated with MPV(P<0.01); SNP3 was significantly associated with mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) (P<0.05); Ins9 was significantly associated with mean corpuscular volume (MCV) (P<0.05) and MPV (P<0.01); SNP10 and SNP11 were significantly associated with MCV, MCH, MPV (P<0.01) and RDW_SD (P<0.05); meanwhile, SNP10 was significantly associated with MCHC (P<0.05). These novel mutations are potential important polymorphic loci affecting the routine blood traits in pregnant sows. The results suggest that the SLA-DOB and CD4 genes as well as their genetic mutations can be as important candidate genes and key molecular markers affecting immunity of pregnant sows.

中图分类号:

S828.2

张丰霞, 杨婷, 敖红, 谭振, 邢凯, 翟丽维, 俞英, 王楚端. SLA-DOB及CD4基因新突变显著影响长白妊娠母猪的血常规性状[J]. 畜牧兽医学报, 2017, 48(11): 2046-2058.

ZHANG Feng-xia, YANG Ting, AO Hong, TAN Zhen, XING Kai, ZHAI Li-wei, YU Ying, WANG Chu-duan. Novel Genetic Mutations in Porcine SLA-DOB and CD4 Significantly Affect the Routine Blood Traits of Pregnant Landrace Sows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2046-2058.

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